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Insert sizes ranged between 150 and 3939 bp, with a majority in the range of 500 1000 bp.
For a potential mSFP call, a conservative signal cutoff ratio of 3 5.0 and a vote among several adjacent probes (within 25 bp) with a majority of the probes (at least 75%) should be applied.
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Analysis of the CpG density for these sperm DMR is shown in Figure 2a and indicates a density <13 CpG/100 bp with the majority of DMR having 1 to 8 CpG/100 bp density.
After trimming and vector and contaminant removal, 10,185 high-quality sequences were obtained with an average read length of approximately 600 700 bp, with the majority of reads being > 700 bp.
A brief survey of published RH maps indicates that PCR-based markers tend to fall in the range of 75 400 bp, with the majority ranging from 100 250 bp.
All samples were sheared to the same extent, ranging from 100 - 500 bp with the majority of fragments at 200 bp.
Average read length after trimming for each library was approximately 600 700 bp with the majority of reads compiled from all 13 libraries being between 700 and 800 bp.
The mean length of these predictions is 3665 bp, with the majority (82.81 %) being shorter than 5 kb (Additional file 1: Figure S12).
Circulating DNA in plasma, however, is known to be highly fragmented with a majority of the fragments being shorter than 500 bp [1], [3].
Sequencing of the region containing Y2 I-AniI target site from cells having lost GFP expression revealed a series of genetic mutations, the majority being small deletions (64.9%≤30 bp), with a lesser number of larger deletions (4%, some over 200 bp); insertions (8.1%) or a combination of insertion/deletions (10.8%) (Fig. 4d, GenBank accession numbers: HQ416600 to HQ416674).
The distribution of the lengths of the theoretically generated MluI/NlaIII fragments is presented in Additional file 4. The majority of fragments are shorter than 1000 bp, with a frequency peak at 50 150 bp.
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CEO of Professional Science Editing for Scientists @ prosciediting.com