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All nested amplicons of 501 bp were restricted into two fragments of 310 and 191 bp by the Hind III restriction enzyme.
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The eaeA amplicon (384 bp) was restricted with EcoR1 and yielded fragments of 305 and 79 bp.
The afaBC3 amplicon (793 bp) was restricted with Hinf1 and yielded fragments of 565 and 228 bp.
All amplified PCR products were restricted into 3 segments of 435, 303, and 144 bp.
Flights were restricted into LAX for six hours.
Only the fragment originating from the Ws allele was restricted into 249 and 17 bp fragments.
Only the fragment originating from the Col allele was restricted into 250 and 30 bp fragments.
The detected genes were confirmed by restriction analysis: sfaDE and papC amplicons (410 and 328 bp respectively) were restricted with HaeIII and yielded fragments of 209 bp, 135 bp and 66 bp; and 161 bp, 112 bp and 55 bp respectively.
Results were similar after controlling for baseline BP and when analyses were restricted to those with baseline BP > 140/90 mmHg.
Subsequent analyses were restricted to the first 397 bp of the D-loop spanning the first hypervariable region (HVR I).
Windows were restricted to the 30 largest scaffolds (903,936 – 2,025,234 bp) from the European eel draft genome.
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CEO of Professional Science Editing for Scientists @ prosciediting.com