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Sequences shorter than 50 bp were removed using MOTHUR v.1.22.2.
Small fragments (<100 bp) were removed using AMPure Beads XP (Beckmann Coulter GmbH, Krefeld, Germany).
The small fragments (less than 100 bp) were removed using AMPure beads, and the supernatant contained the cDNA library.
Finally, the cDNAs shorter than 500 bp were removed using Ampure Beads according to the manufactures' instruction (Beckman, USA) before the preparation of the cDNA library.
After digestion with SfiI, products smaller than 500 bp were removed using the Chroma Spin-400 column column as described in the Creator SMART protocol.
After digestion of the amplified cDNA with the SfiI restriction enzyme, products smaller than 300 bp were removed using the Chroma Spin-400 column as described in the Creator SMART™ protocol and cloned into pDNR-Lib cloning vector.
Similar(51)
FASTA format sequences are extracted from FASTQ files and those<400 base pairs (bp) in length (or<350 bp for the 18 S amplicon) or containing N's or homopolymer runs of>8 bp are removed using MOTHUR, (v1.34.1 20,21.
Subsequently, low-complexity, low-quality, and very short (<50 bp) sequences were removed using Lucy (version 1.20p) (http://www.jcvi.org/cms/research/software/) and the trimmed sequences were assembled using Newbler (version 2.5.3) (http://my454.com/products/analysis-software/index.asp).asp
Short sequences (< 100 bp in length) were removed using custom Perl program [ 55].
PCR products less than 300 bp in length were removed using the PureLink™ PCR Purification kit (Invitrogen).
Sequence reads were trimmed to a length of 90 bp, and adaptors were removed using the cutadapt program [ 26].
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