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Approximately 7.7 million sequencing reads (6.3 billion bp) were produced from the first phase of the GOS expedition.
Of the 22 microscopically positive smears for Leishmania species, amplicons (∼350 bp) were produced from 17 samples (77%).
A total of 365,116 reads (testis 171,962, ovary 193,154) with an average sequence length of 285 bp were produced from testis and ovary cDNA libraries.
In total, 1,596,258 pair-end reads of 250 bp were produced from genomic DNA isolated from the L. newyorkensis isolate using the Illumina MiSeq platform with average insert size estimated at 650 bp.
Briefly, amplicons of ≈830 bp were produced from each sample by using the nested primer set described above (23 ), and an ≈298-bp fragment was sequenced (Genetic Research Instrumentation, Braintree, UK) by using the forward primer 5′-AGTGACAAGAAATAACA ATACAGG-3′ and the reverse primer 5′-CCTGCTTTAAGCACTCTAATTTTC-3′ (24 ).
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In total, 1,341,131 pair-end reads of 250-bp were produced from L. fleischmannii genomic DNA using the Illumina MiSeq platform.
Short reads of 39-bp were produced from Applied Biosystem's (ABI) SOLiD (Sequencing by Oligonucleotide Ligation and Detection) System, and mapped to a reference genome by Life Technologies using SOLiD™ BioScope™ Software, allowing two mismatch.
Paired-end libraries with nominal insert sizes 180 bp and 500 bp were produced.
Paired-end reads of 2 × 100 bp were produced.
The 110 to 140 bp size selected DNA fragments were produced from a series of restriction digestions performed on the 300 to 350 bp DNA fragments, first tier restriction, of both the Jalo EEP 558 and BAT 93 genotypes.
The 1170 bp amplicon was produced from both McrBC-digested and undigested genomic DNA from 1 3 DAF seeds suggesting a lower degree of methylation within region 1 in 1 3 DAF genomic DNA.
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