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The 3'-RACE and 5'-RACE of the resulting fragment of ca. 600 bp were performed with the primers P18 P21.
Analyses of systolic and diastolic BP were performed with linear regression.
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DNA sequencing for the full length of jhp0045 1,0322 bp) was performed with several primer pairs located at jhp0044 and jhp0046.
Cluster generation and sequencing (50 bp) was performed with the hiSeq Solexa Genome analyzer according to standard Illumina protocols.
Paired-end sequencing of 110 bp was performed with the GA, and the fluorescent images were processed to sequences using the Illumina GA Pipeline Analysis software 1.8.
Paired-end sequencing (2 × 250 bp) was performed with MiSeq v2.2 chemistry, and a mean depth of 938 reads/base within the regions of interest was obtained.
Cluster generation and sequencing (36 bp) was performed with the Illumina Genome Analyzer IIx (GAIIx) platform according to standard Illumina protocols.
Paired-end sequencing of 100 150 bp was performed with the Illumina machine using clustered template DNA and the robust four-color DNA sequencing-by-synthesis technology.
Sample preparation, cluster generation and sequencing (36 bp) was performed with the Illumina Genome Analyzer IIx (GAIIx) platform according to standard Illumina protocols.
Likewise, DNA sequencing for the full length of jhp0046 (783 bp) was performed with several primer pairs located at jhp0045 and jhp0047.
Long PCR (amplicon > 500 bp) was performed with the Expand Long Range Kit (Roche, Switzerland) in 50 μl volumes (28.5 μl double-distilled water; 10 μl buffer; 0.5 mM dNTP-mix; 0.3 μM of each primer; 4 μl 100% DMSO; 1 μl template DNA and 1 μl enzyme-mix).
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