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After removing the low quality reads and chimeras, a total of 60,411 chimera-free high quality 16S rRNA gene sequences (V4 533 786 bp) were obtained, with an average of 5034.25 ± 178.35 sequences per sample, ranging from 4038 to 5321.
Anthropometric measurements and blood pressure (BP) were obtained with standard methods as elsewhere described (7).
Then, 89 scaffolds (>500 bp) were obtained with an N50 value of 550,408.
Mate-paired sequence reads of 100 bp were obtained with an average distance of 3000 bp between pair reads.
Read-lengths of up to 36 bp were obtained with per-base accuracies on the order of 99%.
In total, 22,756 unigenes with an average length of 485 bp were obtained with de novo assembly.
Similar(48)
A single amplicon of 789 bp was obtained with the beta-AOB products, but no amplification was observed in both gamma-AOB and MOB (supplementary Fig. 1).
A readable common sequence of 290 bp was obtained with sequence analysis and found to be the same for eight samples, indicating a single outbreak virus strain.
A common band with approximately 330 bp was obtained with all the enzymes used (monomer), and other bands were also observed showing a 330-bp periodicity: 660 bp (dimmer) and 990 bp (trimmer).
For each strain a band of approximately 360 bp and/or of approximately 180 bp were obtained using the 2ab primer set, and a band of approximately 360 bp was obtained with the 2a primer set.
A N50 length of 708 bp, i.e., half of the assembled bases were incorporated into UniGenes with a length at least 708 bp, was obtained, with one third (13,115 UniGenes) having a length over 500 bp (Additional File 2A).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com