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Based on the paired-end information, the contigs (longer than 47 bp) were linked into transcripts.
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Similar in concept to TALEs, eukaryotic zinc fingers (ZFs) are small (∼30 amino acid) modular domains that bind to 3 bp DNA regions with engineerable sequence specificity and can be linked into multifinger arrays that recognize longer sequences.
Shortly, adapted sequences were linked to the generated ChIP, the library was size selected (200 250 bp) and amplified by PCR.
BP lowering was linked to the severity of baseline BP levels.
The first one was linked to the substantial reduction in BP that volunteer had.
Earnings at large oil companies, like BP, are heavily linked to the price of oil, and the recent drop prompted companies to seek ways to increase revenues while lowering costs.
These contigs were further linked into scaffolds by paired-end relationships (300-550 bp insert size), mate-pair reads (2-10 kb), fosmid ends (~40 kb), and BAC ends (~100 kb).
The Warwick group, with its BP Off Campus initiative, is linking up with the national network of anti‐BP groups, and supported protests at the British Museum earlier this month.
The target fragments stx1 (1227 bp), stx2 (1236 bp) and fliC (1758 bp) were ligated into pUC19 vector, and then transformed into E. coli JM109.
ORFs that are larger than 3000 bp were split into smaller fragments with 150 bp overlap for efficient amplification.
Recombinant phages having insert sizes of 200 bp were re-arrayed into new storage plates.
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CEO of Professional Science Editing for Scientists @ prosciediting.com