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Fragmentation was performed in a MJ Research PTC-200 thermocycler at 37 C for 16 min, 99 C for 15 min, and 12 C for 15 min. Samples with similar intensities and fragment sizes (approximate fragment size = 50 bp) were labelled with a master mix of Biotin-N6-ddATP (Enzo) and RTdT enzyme (Promega) to each sample.
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TGF-β1 PCR products (139 bp) were labeled with the fluorescent dye coumarin and hybridized with detection probes on BMPs.
The shearing DNA (200 1000 bp) was labeled with Cy5/Cy3 and hybridized to S. cerevisiae CGH 385 K Whole-Genome Tiling Arrays (NimbleGen).
The 40 bp sequences were labelled with DIG-ddUTP using a terminal transferase (Roche) and hybridized onto dot blots.
The 105 bp DNA was labelled with P at the 5′-ends using T4 PNK.
To test this possibility, RNAs from whole blood, erythrocytes, and leukocytes from the same healthy normal individuals (N = 3) were fractionated and an equal amount of small-sized RNAs (<40 bp) was labeled and interrogated with microRNA microarrays.
A 483 bp E6 probe was labelled with digoxin using an in vitro transcription kit (Boehringer Mannheim, Rocha Diagnostic GmbH, Agency Organisation GD-M Sandhoferstr-116, D-68305 Mannheim, Germany).
To facilitate multiplex genotyping, the expected lengths of the target amplicons were uniformly distributed in the 100 to 450 bp range, and the forward primers were labeled with one of the three fluorophores 6FAM, HEX, and TAMRA.
Oligonucleotide probes of the −160 to −120 bp region sequence in the p57 promoter were labeled with digoxigenin by using a DIG gel shift kit (Roche).
Tester isolates were labeled with Cy5 dUTP.
Cell lines were labeled with Aldeflour reagent.
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