Sentence examples for bp were isolated using from inspiring English sources
DNA fragments in the range of 300 500 bp were isolated using the MinElute Gel Extraction Kit (Qiagen).
Regulatory sequences upstream of the initiation codon of PpNAC2 (of 661 bp) and PpNAC3 (1115 bp) were isolated using a Genome Walking approach.
Samples were then separated by electrophoresis through a 1.5% agarose, 0.5× TBE gel, and DNA fragments from 300 to 700 bp were isolated using a MinElute Gel Extraction Kit (Qiagen).
After the digestion (enzyme MseIwas used), polymerase chain reactions (PCR) and purification of genomic DNA, fragments(with indices and adaptors) of 330 ~ 380 bp were isolated using Gel Extraction Kit (Qiagen) and subjected to PCR amplification (barcode2 was added).
The full length BbKst gene (7375 bp) was isolated using RACE and genome walking.
Samples were then run out on a 1.5% agarose (Sigma), 0.5X TBE gel, and DNA 300 bp to 800 bp was isolated using a MinElute Gel Extraction Kit (Qiagen).
Pooled DNA sample was run out on a 1% agarose (Sigma, USA), 0.5 × TBE gel, and DNA fragments 200 bp to 500 bp was isolated using a Min Elute Gel Extraction Kit (Qiagen, Germany).
The sheared DNA was separated by electrophoresis through a 1.5% agarose gel, and fragments in the 300-800 bp range were isolated using a MinElute Gel Extraction kit (Qiagen).
Following the second strand cDNA synthesis and adaptor ligation, 200-bp and 50-bp cDNA fragments were isolated using gel electrophoresis and amplified by 18 cycles of PCR.
In both cases, 137 bp shRNA products were isolated using EcoRI and XhoI digestion and subcloned into the LMS vector.
