Sentence examples for bp were generated using from inspiring English sources
DNA fragments of 500 1,000 bp were generated using PCR.
An assembly of 66,508 transcripts (>200 bp) were generated using short-read Illumina RNA-Seq libraries constructed from feeding, reproductive, and defensive polyps of H. symbiolongicarpus.
A total of 6,710,574 high-quality paired-end reads of 2 × 36 bp were generated using Illumina GAIIx from the selected cDNA.
Paired-end sequences (101 bp) were generated using three lanes of an Illumina HiSeq2000 flow cell (Ilumina Inc. San Diego, CA, USA).
Single end reads with a length of 100 bp were generated using a HiSeq2000 (Illumina) at the Australian National University (Canberra, Australia).
In parallel, a total of 4 602 723 scaffolds and contigs consisting of 1 301 006 845 bp were generated using SOAPdenovo v1.05 and GapCloser 1.10.
A DNA probe of 1300 bp was generated using the DIG-PCR amplification kit (Roche, Switzerland) with the primers 5'-GAA GAG CCG AAT CCA CAT and-3' and 5'-CTG GCG CTG CTG CCA CTC-3' and the full length human PLEKHA7 cDNA as template.
All sequences were downloaded from GenBank (Sept , 2004 except for SSR sequences kindly provided by Dr. Ben Burr, Brookhaven National Laboratories, NY). Based on the results, a database of primer sets with predicted product sizes of 600-800 bp was generated using Primer3 [ 41] to design primer pairs in the 3' or 5' termini of unigenes from G. arboreum ESTs [ 42].
A total of 8,862,831 single-end reads with an average length of 33 bps were generated using the Solexa Genome Analyzer (Illumina, Little Chesterford, Essex), giving a 63.9× coverage.
The library was prepared using TruSeq RNA Library Prep Kit (Illumina, San Diego, CA), omitting the polyA enrichment step, and the library was enriched for 175 nt fragments so that paired end reads overlapped by 30 nt. 130 million 100 bp read pairs (36 Gb) were generated using the Illumina HiSeq 2000 platform.
Consensus sequences for the SNP positions and 60 bp on either side (121 bp total) were generated using a custom Perl script (SNPseqRetrieve.pl, http://wiki.bioinformatics.ucdavis.edu/index.php/SNPseqRetrieve.pl), followed by BWA alignment to align these candidate SNP sequences to the 'Lovell' sequence ftp://ftp.jgi-psf.org/pub/JGI_data/phytozome/v7.0/Ppersica/.
