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Recombinant Fabs against the non-collagenous 16th-A domain of type XVII collagen, the main pathogenic epitope for autoantibodies in BP, were generated from antibody repertoires of BP patients by phage display.
A total of 15,376 and 10,133 contiguous assemblies ranging in size from 94 bp to 5719 bp were generated from November and June, respectively (Table 1).
A total of 71837 mated sequencing reads (average trimmed sequence read length of 800 bp) were generated from the five sites with an average of 14367 reads per site.
Over 4.8 million high-confidence composite sequences of 180±40 bp were generated from a single sequencing lane of the Illumina GAIIx while 673,673 454-FLX reads of 207±71 bp were already available [10].
MtDNA COI sequences (632 bp) were generated from 17 strains represented by 91 individuals.
PCR products of the expected size (approximately 500 bp) were generated from all 76 isolates.
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The probe, containing the entire orf21 sequence of 519 bp, was generated from the M-type mitochondrial whole genome sequence present in GenBank (AB065374).
One whole genome shot gun sequencing library with insert size of 350 bp was generated from DNA of the pooled samples.
A total of 125,813,174 and 129,412,231 paired-end (PE) reads (2x100bp) with an average insert size of 153 bp ± 39 bp was generated from libraries 120 and 120E, respectively.
A minimal cab promoter was obtained as follows: A PCR product (560 bp) was generated from a genomic DNA template using the Universal AP2 primer (Genome Walker kit) and a gene-specific primer, cabpro570, containing a built-in BamHI site (5'-ATGTATCAAT GGATCCTAATGAGATGCC-3').
Thus, if the first BPs are generated from Tis21-nucGFP positive APs, Tis21-nucGFP positivestrAPshthiswouldexprovideon why newborn BPs lose apical contact, retract their apical procestraightforwardexplanationapical-basal polarity at mitosis (Fig. 8A).
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