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Entry clones (in donor vector, pDONR™222) with constructs of sixteen "unknown" gene fragments in the range of 700 1800 bp were developed using standard procedures.
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Analysis pipelines were developed using the 75 bp fragment reads for the Angus steer.
In the present study, PCR-based InDel markers were developed using 80 to 200 bp PCR products, and the insertion/deletion size varied between 4 to 10 bp.
Membranes were developed using ECL Substrate.
Primers were developed using Primer Express version 1.5 (Applied Biosystems Inc., Foster City, USA), using the following parameters: Minimum Tm: 59 60°C, Maximum Tm difference between primers: 1°, Oligo length: 20 25 bp, Amplicon length: 90 120 bp.
A novel design of copper metal bipolar plates (BPs) was developed as well as fabricated in-house and used in this fuel cell stack.
A new particle beam technique using Ar BPs has been developed and used to remove CPs of 20-nm size from flat surfaces and trenches [12, 13]; its success was attributed to the nanometer size and supersonic velocity of the BPs.
A multiplex PCR was developed and used for simultaneous detection of blaCTX-M (551 bp) and blaTEM (972 bp) genes.
BP defended the use of the Corexit products, mainstays of oil spill control that were developed in the 1980s and '90s.
To address these environmental issues, biodegradable plastics (BPs) have been developed (Gross and Kalra [2002]).
After removing redundancy, the remaining unique sequences that had 3′UTR sequences longer than 100 bp were used to develop HAU-InDel-prefixed markers to amplify InDels existing in the 3′UTRs.
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