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Products of 573, 596 and 766 bp were detected using specific primers of plnA, plnEF and bre174A, respectively.
Deletions ≥250 bp were detected using combined paired read data and localised homology mapping across the deletion boundary.
CpG islands of at least 200 bp and with a GC percentage exceeding 50% and with an observed/expected CpG ratio that is greater than 60 bp were detected using the FIND-CPG program from the PipTools [23].
Other small indels (<100 bp) were detected using the software Pindel [ 84].
Short tandem repeats (2 – 5 bp) were detected using Tandem Repeats Finder [ 46].
Minisatellites (repeat units usually within 7~2000 bp) were detected using the program Tandem Repeat Finder 4.03 [ 22].
Similar(52)
Gene symbol identities corresponding to the four different lists of seed-proteins were loaded into the ExPlainTm 2.3 Tool [ 36], where functional groups of Gene Ontology Biological Processes (GO-BP) were detected using a p-value threshold of 0.05 as the classification criteria and one as the minimal number of genes assigned to a group (i.e.: number of hits).
Genomic variants (single-nucleotide variants and short indels [1 15 bp]) were detected using the Genome Analysis Toolkit (GATK version 1.0.6001) (DePristo et al., 2011; McKenna et al., 2010).
The reaction was stopped by adding the Dicer stop solution and 22 bp products were detected using 3% agarose gel electrophoresis [ 37].
Briefly, from previous BP and HR recordings, all up (increase in BP and decrease in HR) and down (decrease in BP and increase in HR) baroreflex sequences were detected using Hemolab software (http://www.harald.nxserve.net/HemoLab/HemoLab.php) and the average SBRS was calculated.
Minisatellite (10 99 bp, repeated at least 2.9 times) and satellite (>100 bp, repeated at least 2.9 times) sequences were detected using TRF [ 60] and clustered by cd-hit [ 61].
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