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Bands of 715 bp were cleaned with a QIAGEN gel extraction kit before DNA sequencing (Davis Sequencing, Davis, CA, USA).
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The PCR products (341 bp) were cleaned using a Qiagen MinElute purification kit prior to analysis with Bioanalyzer (Agilent).
Cloned PCR products between 200-800 bp were cleaned as above.
Nested PCR products (900-1,200 bp in length) were cleaned with the Qiagen PCR Purification kit and sequenced on an ABI 3130 × l sequencer.
The chambers were cleaned with 70% isopropanol.
The hind legs were cleaned with iodine.
The components were cleaned with saline solution.
All Sanger reads were cleaned with Seqclean [ 67].
Tissue samples were cleaned with xylene.
These lesions were cleaned with 70% alcohol.
Fragmented DNA was cleaned with AmpPure XP beads to remove fragments < 100 bp.
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