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Resultant rpoB sequences (~ 730 bp) were analyzed using MEGA version 5.0 software [ 31].
Total snoRNA probes with different lengths (48 237 bp) were analyzed using an outlier analysis (Supplementary data; S3A).
PCR products of 223 bp were analyzed using electrophoresis on a 2% agarose gel, stained with ethidium bromide and observed under UV light.
The similarities of the sequences from reamplified and cut DGGE bands (approximately 550 bp) were analyzed using the NCBI Blast search tool at.
Sequence contigs > 1,000 bp were analyzed using a variety of sequence similarity searches and gene prediction algorithms that have been incorporated into an in-house computational pipeline and database [ 35].
The re-aligned teleost PDE1C genes (1248 bp), excluding the partial zebrafish PDE1Ca (1185 bp), were analyzed using the ML method under the TrN + Γ model of nucleotide substitution [ 50], which was chosen by the hLRT.
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The third fragment, from adiponectin gene (518 bp) was analyzed using PCR-RFLP (data not published yet).
Based on the results of the HD array an area from 33,201,457 bp to 34,393,006 bp was analyzed using sequence data (Additional file 4).
A total of 1551 bp sequence were analyzed using maximum likelihood (ML), maximum parsimony (MP) and neighbour-joining (NJ) methods in PAUP* 4.0b10 [ 36].
The PCR fragments were transferred to Zetaprobe blotting membrane through capillary action with 0.4 N NaOH, and the intensities of the 250 bp radioactive bands were analyzed using the Storm 860 Phosphor Imager software (GE Healthcare).
BRCA1 and BRCA2 coding homopolymers of 6 bp or longer were analyzed using the BRCA HP v.2.0 (Multiplicom).
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