Sentence examples for bp were amplified using from inspiring English sources
ITS1 fragments of approximately 400 bp were amplified using Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc).
Partial 16S rDNAs (~640 bp) were amplified using actinomycete-specific primers Act-0235 (5′CGCGGCCTandAGCTTGTTG3′) and Act-0878 (5′CCGTACTCCCCAGGCGGGG3′) under previously established conditions (Stach et al. 2003).
Exons>400 bp were amplified using overlapping primer pairs.
Two bands of the predicted size (~500 bp) were amplified using the genomic DNA of pepper (Additional file 1).
Targeting flanking sequences TS1 (572 bp) and TS2 (527 bp) were amplified using primer pair P1 and P2 and primer pair P3 and P4.
For the quantification of the 4435A>G mutation, the PCR segments (700 bp) were amplified using genomic DNA as template and oligodeoxynucleotides corresponding to mtDNA at positions 3861 4560, and subsequently digested with a restriction enzyme NlaIII.
A single fragment of 270 bp was amplified using degenerate primers DFP and DRP from the genomic DNA of Penicillium sp. D-1.
The partial histone H3 gene fragment with size of 223 bp was amplified using primers H3dStyI (5′-AGCATCACCYGAACATCGCATCATCCCATG-3′) and H3R1 (5′-TTGGACTGG-ATRGTAACACGC-3′), and purified with Plus DNA Clean/Extraction Kit (GeneMark, Taichung, Taiwan).
For the rbcL gene, ca. 1400 bp was amplified using the primers F1 and 1379R [53], and sequencing reactions were performed using combinations of four primers: F1, 645F, 888R and 1379R [54], [55].
The COI minibarcode (130 bp) was amplified using a commonly used forward primer LepF1: 5'-ATTCAACCAATCATAAAGATATTGG-3' [23] and a newly designed reverse primer, EPT-long-univR: 5'-AARAAAATYATAAYAAAIGCGTGIAIIGT-3' in a two-step PCR amplification regime.
A fragment (480 bp) was amplified using the primers pE2* (5'-CAA CCG AGC TGA AGC ATT CTG CCT /X2 (5'-CCT GCT CAC GGC GCT CCC CAG CAT G) containing sequence information from Exon3 to codon 132/133 of the murine PrP.
