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LS mean changes (95% CI) from baseline in systolic BP at week 52 were −3.4 mmHg (−5.5, −1.4) and −3.7 mmHg (−5.8, −1.6) with canagliflozin 100 and 300 mg, respectively (Table 2); reductions in diastolic BP were also seen.
Greater reduction in body weight was observed with canagliflozin relative to sitagliptin (difference of −2.8%[−2.44 kg]); reductions in FPG and systolic BP were also seen with canagliflozin vs sitagliptin.
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Spindle defects were also seen.
For sex identification, an individual was assigned as female if the 104-bp band was exclusively seen in four positive PCRs at the amelogenin locus, while the status of male was assigned if the 110-bp band was also seen in at least two positive PCRs.
If long TSDs are a mark of a young SINE we would expect the 15 "genuinely recent" EhSINE1s to have longer TSDs than a random SINE and this is also seen: 21 ± 5 bp v 13 ± 8 bp for the whole set.
In contrast to the GL-1 ITD, which produced only one > 500 bp amplicon, the wild-type FLT3 allele was also seen in these canine ALL blood samples, either indicating heterozygosity or contamination with non-leukemic cells.
Similarly, strong depletion with gradual approach to 1 is also seen for all 83-bp AT-rich control sequences.
A second spurious band is also seen in lane 6 at 381 bp.
SNP loci with an additional SNP within 7 bp were also discarded (but see Additional file 3) to avoid possible artifacts of slight alignment errors.
Based on the sequence alignment and domain analyses, a truncated form of Af1 (1614 bp), full length Af2 and a truncated form of Af3 (1632 bp) were also cloned into a pCRE2 vector by using the In-Fusion PCR Cloning kit (Clontech) (see online resource for details).
Reads < 40 bp were also discarded.
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