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Thirty-four exonic microdeletions of 30 bp were also generated to map splicing regulatory elements.
In a similar manner, simulated paired-end reads of lengths 76 and 35 bp were also generated with error profiles based on datasets ERR007826 and ERR000408, respectively.
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A set of simulated alignments, i.e. 26 alignments with length around 100,000 bps are also generated according to the frequency and the conservation of the nucleotides in the alignment of potential non-exons.
For Te. subulata, paired end reads of 100 bp in length were also generated on the Illumina HiSeq platform, producing 176,799,164 raw reads with 92.42% having a quality score of Q30 or better, with no read trimming deemed necessary.
Two fragments of 150 bp and 170 bp respectively from diploid G. herbaceum and G. raimondii were also generated.
RNA interference (RNAi) lines that express a 302-bp inverted-repeat sequence of OsWRKY80 coding region were also generated.
Three fragments, each of approximately 500 bp, spanning three independent regions of the β-actin transcript were also generated.
GC % and GC skew maps were also generated in Genomeviz, with a window size of 1000 bp, and an overlap of 500 bp between windows.
The next observation we made was that a subclass of putative centromeres containing 147-bp tandem repeats were also generating siRNAs [ 17].
A GFP ORF, flanked by two 34-bp minimal FRT sites, was also generated by using pEGFP-N1 (Clontech) as a template to obtain pMY151.
In D. pseudoobscura, the polymorphic inversion Arrowhead and a number of fixed inversions have been also generated by ectopic recombination between 128-bp and 315-bp repeats, yet the nature of these repeats is obscure [39].
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