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The resulting fragment of 4,385 bp was sequenced using the latter primers as well as internal primers.
The final extension at the end of the profile was at 72°C for 10 min. Part of exon 1 of IRBP (ca 1270 bp) was sequenced, using the methods of Poux and Douzery [ 121].
A BAC clone CHORI82_R4_266G10, which spans the genomic region of ADAM23 on CFA37 (at 14898298 15055982 bp) was sequenced using PacBio sequencing technology (Pacific Biosciences, Menlo Park, CA, USA) at DNA Sequencing and Genomics laboratory, Institute of Biotechnology, University of Helsinki, Finland.
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The amplicons (893 bp) were sequenced using an automated sequencer by the Sanger method (ABI Prism 377 sequencer; Perkin-Elmer) [ 10].
Appropriately-sized PCR products (413 bp) were sequenced, using a CEQ8000 DNA sequencer (Beckman-Coulter, Fullerton, CA).
The 16S rRNA PCR products obtained (after purification, type I – 700 bp, type II – 530 bp) were sequenced using genomic DNA from Sphagnum mosses as a template.
The complete mitochondrial control (1,094 bp) and cytochrome c oxidase subunit I (COI) region (1,544 bp), as well as a region of the melanocortin 1 receptor gene (57 bp) were sequenced using a multiplex PCR approach.
Subsequently, PCR-amplified fragments of approximately 180−350 bp were sequenced using Illumina Genome Analyzer.
The short amplified products (smaller than ∼1,500 bp) were sequenced using the amplification primers.
Inserts with a size between 300 and 700 bp were sequenced using BigDye Terminator version 3.1 (Applied Biosystems, Foster City, California, USA).
Paired-end libraries of 800 bp and 400 bp insert lengths (for the race Ac2V only one library of ∼400 bp) were sequenced using Illumina Genome Analyzer II platform at the Sainsbury Laboratory Sequencing Centre (GA2).
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