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kyr BP was obtained from Lake Kushu.
A sequence of 1614 bp was obtained from cucumber genomic sequence, which consists of two exons and one intron.
Through the combinatorial usage of three primers with a single reaction set, two fragments of 290 and 159 bp were amplified from the cattle meat DNA, whereas only a fragment of 290 bp was obtained from the yak meat DNA.
pSP72 (2462 bp) was obtained from Promega (Madison, WI).
Using degenerate primers designed on the basis of conserved domain of insect deduced SID-1 amino acid sequences, a partial sequence (231 bp) was obtained from S. exigua by RT-PCR, and a longer 939 bp sequence was isolated using 3'-RACE with the specific primers on the 231 bp PCR fragment.
In addition, a human insulin gene (approximately 1,800 bp) was obtained from human genomic DNA.
Similar(39)
Amplicons of 450 bp were obtained from all Fusarium isolates, while no PCR product was obtained from non-Fusarium organisms.
A total of 2,945,166 sequence reads with a median length 450 bp were obtained from all fecal samples.
After two rounds of amplification, fragments ranging from approximately 500 1500 bp were obtained from the 10 adhesion- or invasion-defective mutants.
Approximately 49,722,976 reads with average 114.9 bp and 29,726,302 reads with average 111.7 bp were obtained from two independent libraries of leaf tissues, respectively, and 21,207,630 reads with average 95.8 bp in length were obtained from floral tissues (Additional file 1: Table S1).
The MUC2 genomic DNA sequences (37,982 bp) were obtained from the UCL website [21] and from NCBI (AC139749.4).
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