Sentence examples for bp was generated with from inspiring English sources

The largest product (1745 bp) was generated with HincII digested DNA.

18S rRNA gene fragments of approximately 500 bp were generated with the following protocol: initial denaturation at 95°C for 5 min; 8 cycles of 94°C for 30 s, 55°C for 30 s and 72°C for 1 min; 20 cycles of 92°C for 30 s, 52°C for 30 s and 72°C for 1 min; followed by final extension at 72°C for 10 min.

Since DNA fragments of 500 1000 bp were generated with sonication, epigenetic changes in the distal enhancer region of the Pou5f1 promoter were detected with primer pair A (−2281 to −2212 relative to TSS), and changes in the proximal enhancer/proximal promoter region with primer pair B (−854 to −781 relative to TSS) (Figure 3A).

The single-end reads with the size of 75 bp were generated with our in-house SOLiD 5500 platform.

As a result, 164,874 contigs (> 200 bp) were generated with an average length of 621 bp and a N50 of 927 bp.

The probe (402 bp) was generated by PCR with primers GUS FII (5′-CAGTCTTACTTCCATGATTTCTTTA-3′) and GUS RII (5′-AGTAAAGTAGAACGGTTTGTGGTTA-3′).

The fragment with 921 bp was generated by exonuclease digestion.

Finally, each cDNA library with an insert size of 200 bp was generated.

A 535-bp amplimer was generated with primers MTLa1-F (5′-TGAAAATGAAGACAATGCGA-3′) and MTLa1-R (5′-CATCTTTTTCTGCTATCAATTC-3′) in the presence of MTL type a DNA, and a 615-bp product resulted from primers MTLα1-F (5′-ATGAATTCACATCTGGAGGC-3′) and MTLα1-R (5′-CTGTTAATAGCAAAGCAGCC-3′) in the presence of MTL type α DNA.

As described in the Method section, a 259 bp PCR product was generated with a second set of PCR primers that would specifically encompass a differential Bgl1 restriction enzyme cut site present in CaT1 but not ECaC1.

One paired-end library with a 450-bp insert size was generated with the NEXTflex PCR-Free DNA Sequencing Kit (Bioscientific) and sequenced on one lane of the HiSeq 2000 platform (Illumina) with Illumina TruSeq V3 Kit (200 cycles).This has been submitted to the NCBI Sequence Read Archive under the accession number SRP055078.