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The PCR product (376 bp) was digested with NdeII to differentiate between Helicobacter heilmannii and Helicobacter pylori.
Fragment 5 (3610 bp) was digested with EcoRI which generates predicted products of 1531 and 2079 bp.
Fragment 4 (3280 bp) was digested with Sau96I which generates predicted products of 186, 336, 455, 584 y 1719 bp.
Fragment 2 (2456 bp) was digested with SspI which generates predicted products of 56, 359, 530, 1511 bp.
Fragment 1 (2800 bp) was digested with Sau96I which generates predicted products of 16, 151, 419, 712, 1502 bp.
Fragment 3 (3503 bp) was digested with BamHI which generates predicted products of 628, 636, 2239 bp.
Similar(42)
PCR products (756 bp) were digested with Kpn I and Hind III and ligated into pGem- YlCWP1 digested with the same enzymes.
The amplified DNAs of pcaIJ (1,404 bp), pcaFD (1,998 bp), and pcaHGBC (2,799 bp) were digested with Pst I or Nsi I, respectively, and were further ligated with Pst I-digested pSL360 (same overhang with Nsi I digestion) resulting in pSL360-pcaIJ, pSL360-pcaFD, and pSL360-pcaHGBC, respectively.
After PCR amplification, the LINE-1 amplicons (160 bp) were digested with TaqI and TasI in NEB buffer 3 (New England Biolabs, Ontario, Canada), while the Alu amplicons (117 bp) were digested with TaqI in TaqI buffer (MBI Fermentas, Burlington, Canada).
Plasmids with mmr inserts too long to be fully sequenced by Sanger sequencing (approximately >1500 bp) were digested with EcoRI to isolate the insert.
The PCR products (413 bp) were digested with HinfI restriction enzyme (Fermentas), which only recognizes and cuts repetitive elements that are originally methylated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com