Sentence examples for bp was amplified using from inspiring English sources
The pre-miR-411 (96 bp) was amplified using H1299 genomic DNA and cloned into the pLenti vector (Invitrogen) after double digestion by BamH I and Xho I, forming pLenti-miR-411 and sequenced (Sangon Biotech, Shanghai, China).
A mtDNA control region fragment of 683 bp was amplified using two primers developed by Randi et al. (1998): Lcap Pro 5′-CGTCAGTCTCACCATCAACCCCCAAAG-3′ and Hcap Phe 5′-GGGAGACTCATCTAGGCATTTTCAGTG-3′.
A single fragment of 270 bp was amplified using degenerate primers DFP and DRP from the genomic DNA of Penicillium sp. D-1.
The partial histone H3 gene fragment with size of 223 bp was amplified using primers H3dStyI (5′-AGCATCACCYGAACATCGCATCATCCCATG-3′) and H3R1 (5′-TTGGACTGG-ATRGTAACACGC-3′), and purified with Plus DNA Clean/Extraction Kit (GeneMark, Taichung, Taiwan).
To construct a spoT-3x-flag-kan fusion, a C-terminal spoT gene fragment (510 bp) was amplified using SpoTC-1 and SpoTC-2 primers and cloned into HindIII and BamHI sites of pUC18 to construct pYA4642.
For the rbcL gene, ca. 1400 bp was amplified using the primers F1 and 1379R [53], and sequencing reactions were performed using combinations of four primers: F1, 645F, 888R and 1379R [54], [55].
Crude eDNA was then spin-column-purified (PowerMax soil DNA kit) and employed as a template in PCR experiments targeting ADs as follows: AD fragments (~795 bp) were amplified using primers: 5′-GCSTACSYSATSTACACSTCSGG-3′ and 5′-SASGTCVCCSGTSCGGTA-3′.
Three 5′-flanking regions of the rat Vegf gene; 1) the construct including both C/EBPβ binding site and HIF1α binding sites (−1171 bp to +115 bp), 2) the construct lacking the C/EBPβ binding site (−976 bp to +115 bp) and 3) the construct that four HIF1α binding sites are mutated (−1171 bp to +115 bp), were amplified using the primer sets in Table 1.
ITS1 fragments of approximately 400 bp were amplified using Phusion High-Fidelity DNA polymerase (Thermo Fisher Scientific Inc).
Partial 16S rDNAs (~640 bp) were amplified using actinomycete-specific primers Act-0235 (5′CGCGGCCTandAGCTTGTTG3′) and Act-0878 (5′CCGTACTCCCCAGGCGGGG3′) under previously established conditions (Stach et al. 2003).
