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Roche sequence-reads are 100 611 bp in size (mean 344 bp, standard deviation 108); Illumina sequence-reads are all 90 bp in size.
Results A significant positive correlation between the extent of renal scarring and systolic and diastolic BP standard deviation score (SDS) was found by using ABPM.
Allele sizes were determined with the Quantity One software version 4.6.3 (Bio-Rad) through comparison with the 10 bp standard weight marker (Invitrogen™).
Resultant indel lengths averaged 1.84 bp (standard deviation (sd) = 0.02).
The aligned mean insert size was 188.76 bp (standard deviation = 44.81 bp) for males and 167.62 bp (standard deviation = 37.29 bp) for females.
The fragment size for each locus was determined by 100 bp standard size markers (NEB).
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Paired-end sequencing reads were mapped to the genome with TopHat (version 1.2.0) (Qian et al. 2010; Xiong et al. 2010; Chang and Liao 2011), allowing a 100-bp standard deviation for a mean inner distance of 370 bp between paired reads from both ends.
The alleles were sized according to 50- to 350-bp standards (MWG Biotech AG, Ebersberg, Germany).
The mean distance between the nearest SNP markers was found to be 102 bp with standard deviation of 502 bp, median distance of 22 bp, and largest distance of 393 Kb.
Thus the probe equally recognizes the 900 bp internal standard pcr fragment and the 1 kbp genomic SalI fragment.
Sequences were generated using an average error rate of 0.85% substitutions and 0.15% indels (-m 0.85 0.15), and normal distributed read lengths with a mean of 380 bp and standard deviation of 100 bp (−l 380 normal 100).
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