Your English writing platform
Free sign upSuggestions(5)
Exact(12)
We show that the two expression systems yield mature guide strand sequences that differ by a 4 bp shift.
Incorporating a corresponding 4 bp shift into the guide strand of shRNAmiRs resulted in improved knockdown efficiency of BCL11A.
Interestingly, a multiple alignment of these 4 Fur-box consensus sequences showed that although the E. coli consensus sequence seems more divergent, it perfectly matches the 3 other sequences after a 3 bp shift (Figure 3B and C).
While a previous work reported a 20 bp shift in the positioning of nucleosome +2 of the GAL1 promoter in htz1Δ with a similar approach, this result was obtained with a wild type strain in which Htz1 was tagged with Myc [7].
The 3 bp shift of vvi-miR171b was already observed and deposited in miRBase.
The analysis was performed over the length of the entire gene plus 125 kb flanking on each side, in windows of 100 bp with 25 bp shift increments.
Similar(48)
Increasing EU2dT stem length by two base pairs (EU2+2 bp) shifted cleavage by 2 nt (lane 3).
Insertion of an additional 2 bp (EU2+4 bp) shifted cleavage further by two nucleotides, while strongly reducing cleavage efficiency (lane 4).
The nucleotide diversity π with Jukes-Cantor correction [40], the Watterson's estimator of the population mutation rate per site θ [41] and Tajima D [29] were computed using DnaSP 4.10 [42], using a sliding window of length 100 bp shifted by 25 bp at each step.
During the comparisons of the three databases, we found more than 2,000 exons with 2 bp shifts on splicing donor or acceptor between GASS and RefSeq-rheMac3.
Detailed analysis showed that the 2 bp shifts and incomplete splicing sites were led by the mis-assemblies of the rheMac3 genome.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com