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All upstream 500 bp sequences from the whole genome were used as the background control.
All upstream 500 bp sequences from the Arabidopsis genome were used as background control.
As a result, we finally utilized 6,719 sequences from the first group and 140 (12,694 bp) sequences from the second.
Both partial gltA (341 bp) and ompA (325 bp) sequences from the 2 patients were identical to each other and to those of R. raoultii.
To investigate this, we extracted the flanking 10 bp sequences from the reference genome and visualised this potential ENU motif using WEBLOGO [ 12].
To do this, we generated all possible 36 bp sequences from the reference sequence and mapped them back to the reference using blat (Kent 2002).
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Using specific primers designed on the Map-IS900 sequence and on the M. porcinum sequence determined in this study, a 1385 bp sequence from the M. porcinum genome was characterized.
PCR analysis revealed the presence of a faint band of ~ 2.2 Kb corresponding to the AOX1 gene, and a 1.4 Kb amplification product composed of SAM2 gene and 220 bp sequence from the pPIC3.5 K vector (Figure 1a).
These primers amplify a 374 bp sequence from the pCMV promoter.
Both PCR products contained the same 300 bp sequence from the kusA ORF (later on named direct repeat, DR).
However, BLASTn searches using the MITE consensus sequence against the NCBI databases did retrieve one 1604 bp sequence from the genome of A. castellanii with sequence similarity to the MITE TIRs (coordinates AEYA01001964.1: 92260–93863).
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