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A MER48 LTR is found within 500 bp of the annotated transcriptional start sites of nine Ensembl annotated lncRNAs.
Human and mouse promoter sequences (−2000∼ +500 bp of the annotated transcription start sites) were downloaded from Table Browser of the UCSC genome browser [70].
These five blocks each occur within 300 bp of the annotated start of the downstream gene.
For two of them, the position was far upstream (>5000 bp) of the annotated gene and thus not further investigated (OR10J7P and OR4C15).
Specifically, we considered a gene to be TATA-containing if it contained a motif hit with score ≥ 6 within 250 bp of the annotated TSS position.
For transcription levels, we calculated RPKM using GROseq reads from 500 bp of the annotated start site to the annotated termination site in order not to include transcriptional pausing at promoters [ 20, 48].
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For all genes the region up to 1,500 bp upstream of the annotated gene start was used as the proximal promoter region.
The same for the pause peak increase and decrease, but using a 250 bp region with maximal read density in a window of ±500 bp around the annotated TSS.
The significant lower number of tag pairs at the protein coding region in the RL-SAGE library is likely because that the majority of the RL-SAGE tags were located in the 500 bp downstream region of the annotated genes.
Here truncated from original format "HM000... .......... Total bps: total length, in basepairs, of the annotated gene.
These were cut-and-paste into random locations in the genome (excluding regions within 100 bp of already annotated TEs and Ns).
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