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In C. resedifolia, the mean length was 592.2 ± 5.8 bp (median = 528 bp; mode = 384 bp).
In C. impatiens, the mean length was 592.3 ± 5.8 bp (median = 528 bp; mode = 384 bp).
The genomic libraries were sequenced with the Illumina HiSeq system using the paired-end 100 bp or 151 bp mode.
Sequences were assembled into 65,472 contigs (and 172,108 singletons) with a mean length of 625.9 bp (median = 499 bp; mode = 365 bp).
The 2,922 A. thaliana aligned sequences of our final dataset had a mean ± SE length of 594.0 ± 5.8 bp (median = 531 bp; mode = 369 bp).
The resulting 2,263 STARortholog quintuplets had a mean length ± standard error (SE) of 1,335.7 ± 29.0 bp (median = 1,092 bp; mode = 942 bp), corresponding to 69.9 ± 29.5% of the D. melanogaster gene length.
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The shape of this curve represents separate genomic processes generating an intron size distribution with a broad peak of approximately 500 to 2,000 bp in addition to the usual teleost approximately 80-bp mode size.
Fragments captured in hybridization were indexed, amplified and sequenced in a paired end 100-bp mode using an Illumina HiSeq2000 deep sequencing instrument (v3 sequencing chemistry; Illumina, San Diego, CA).
The zebrafish D. rerio in particular does not have monotonically decreasing intron frequency with size from an approximately 80-bp mode, as the other fish appear to have, but rather has a second peak of introns in the 500- to 2,000-bp 2,000-bp
NO rMAL reports fragment sizes with a mean value of 105 bp and mode value of about 120 bp, whereas TF reports a distribution with mean and mode of about 84 bp.
Pools were sequenced on an Illumina HiSeq2000 in 100 bp paired-end mode.
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