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Moreover, a stop codon TAG 12 bp in the upstream sequence makes the start codon of cemA in date palm rather ambiguous.
Our ChIP/ChAP-seq analysis clearly detected two peaks that are separated by 200 bp in the upstream region of sgmA (Fig. 2B).
Compared to P1-rr, P1-rw1077 has a deletion of 381 bp in the upstream repeat, which causes the loss of cob glume pigmentation [ 13].
However, we failed to discriminate two AdpA-binding sites that are separated by 50 bp in the upstream region of bldA (data not shown).
Finally, all BLAST hits in the cucumber genome, together with flank regions of 5,000 10,000 bp in the upstream and downstream of BLAST hits, were annotated using the FGENESH (http://www.softberry.com/) and GENSCAN (http://genes.mit.edu/genescan.html/) programs.
Re-sequencing of the four parents and comparison with the reference genome sequence uncovered a deletion of 232 bp in the upstream region of ppa007577m that is homozygous in NJ Weeping and heterozygous in Ambra, Bounty and the WxBy F1 parent.
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The polyadenylation signals were searched in the 3'UTR sequence using the PatSearch program [ 69], and looking for a one-base variant of the canonical AAUAAA sequence in the 50 bp segment upstream each experimentally identified polyadenylation site.
Haplotyping of BnFLC.A10 specific markers for indels I IV and SNPs 1 6 confirmed that most winter rapeseeds had a 621-bp insertion in the upstream region of BnFLC.A10 (haplotype I), whereas the 621-bp fragment was absent in the spring types (Table 2).
An application based on the NetBeans Platform (http://platform.netbeans.org) was developed to search for MRE-like motifs in 300 bp of the upstream regions of all iron-regulated genes.
Finally, in tomato, the difference in the transcript levels of Ovate between the two cultivars with the different fruit shape is likely attributed to a 16-bp indel in the 5' upstream region [ 2]; in the pepper cultivars examined here no such difference was observed in the sequences of the 5' upstream region.
The rest of the operons selected met both or either of two criteria: a significant expression difference elicited by the arcA mutation and a 15-bp sequence in the upstream region similar to the E. coli consensus ArcA-P binding motif.
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