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Cross-linked CENP-C had a peak at ∼150 bp in the central domain.
In contrast, comparisons of atp1 from Pilostyles-AZ to atp1 from its host revealed identical sequences for 804 bp in the central region, but 15 differences at the 5' and 3' ends.
In contrast, comparisons of atp1 from Pilostyles (AZ) to the host Psorothamnus individual it was collected from, revealed that the two species were identical for 804 bp in the central region of the gene, but have a disproportionate 15 differences at the 5' and 3' ends.
For cDNA amplification, a region of approximately 620 bp in the central portion of the gag gene was chosen (nt 1,778 2,396 in the HERV-K HML-2.HOM) reference sequence [GenBank accession no. AF074086.2]) tHERV-K HML-2.HOMn 35 (97.22%) of tHERV-K HML-2.HOMoci, including HEreferencend HERV-K115, and 13 sequenceloci with internal sequences between 3,889 and 6,579 bp.
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DNA sequencing of the region revealed a 81 bp deletion in the central part of the glycolytic pykA gene.
QuickChange™ Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA) was used to generate 7 bp-deletions in the central portion of every single TFAP2A binding site in single or multiple combinations and pGL3-ESDN-MUT1, pGL3-ESDN-MUT2, pGL3-ESDN-MUT3, pGL3-ESDN-MUT1,2, pGL3-ESDN-MUT1,3, pGL3-ESDN-MUT2,3, pGL3-ESDN-MUT1,2,3 reporter vectors were obtained.
The DMB2 hot spot was included in the analysis, but the chimpanzee sequence was missing 34 bp from the central region.
The S alleles also have a variable number of 12-bp repeats (5'CACAGAATTAAA) in the central region (Fig. 3A, lower).
yr BP, similar to many other sites in the central Mediterranean region.
To introduce mutations in the second step of SSG and gsSSG, a fragment was amplified using a 20-mer primer, a 40-mer primer containing a single point mutation in the central 20 bp, and the template plasmid, pS240.
DMEA can leverage this fact by focusing on motifs that are enriched in the central 100 bp portion relative to the flanks, of genomic regions identified by ChIP-seq.
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