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In addition, this intron spans ca. 400 bp in the c-fos ortholog from Fugu rubripes, an organism which has a very compact genome with rare introns.
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Due to a repertoire of transcriptional response elements in the c fos promoter, c-fos is regulated in response to diverse extracellular signals [ 30].
Moreover, data from previous studies have shown that the C-fos mRNA expression was significantly correlated with c-Fos protein expression in neurons of brain regions [ 56- 59].
Western blot was used to detect the c-Fos, nNOS, nuclear Nrf2, HO1, and NQO1 expressions in TNC.
Taking this background into consideration, we analyzed DNA methylation in the promoter region of c-fos, but found that the c-fos promoter was unmethylated.
Noteworthy is the observation that in these fos mice another component of the AP-1 family, Fra-1, rescues c-Fos functions in the growth process when its expression is controlled by the c-Fos promoter but fails to substitute c-Fos in inducing expression of target genes in fos fibroblasts [27].
Regional analysis revealed significant increases in c-fos in the cingulate cortex and primary sensory cortical areas.
One of the earliest responses after mechanical loading to bone is an increase in the expression of c-Fos.
In contrast, C-Fos staining was almost absent in the contralateral side (Figs 2C, E).
This system reproduces in several respects the chromatin structure and regulation of the chromosomal c-fos gene.
Under these conditions, the levels of c-fos in the rat amygdala, hippocampus and hypothalamus were not changed.
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