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The largest size variation in introns is seen in intron 1 with variations ranging from 683 bp in fugu to 27,524 bp in frog.
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The sizes of the first two Ugt2a exons are 231 and 490 bp in fugu, and 246 and 490 bp in medaka, respectively, corresponding to the 712∼733 bp in length of the zebrafish Ugt2a variable exons.
CNE density is defined as the number of bases residing in CNEs per unit length (1 kilobase in human; 100 bp in fugu) of non-repetitive noncoding sequence in a human gene locus or the longest orthologous fugu gene locus.
We compared the genomic context of msxb and msxc in Danio and Fugu to that of Msx1 and Msx3 in human and mouse (data not shown).
In order to search gene candidates involved in TTX accumulation in the torafugu pufferfish Takifugu rubripes, a custom 4x44k oligonucleotide microarray slide was designed by the Agilent eArray program using oligonucleotide probes of 60 bp in length referring to 42,724 predicted transcripts in the publicly available Fugu genome database.
The overall gene size varies from 2.9 kb in the condensed genome of fugu to 43.8 kb in frog.
These additional TFs in fugu are likely to be the result of the 'fish-specific' whole-genome duplication in the fish lineage [ 22].
By analysing the genome database of Fugu 20 h/E spl)/ hey genes in Fugu were determined, compared to 10 currently known members of this family in zebrafish.
The isotocin gene in fugu has been translocated to a position upstream of vasotocin gene and the two genes are separated by five unrelated genes.
The CNE density in fugu also ranges widely from 0.02 to 67 bp per 100 bp of fugu sequence (Fig 2).
The mutation frequency in fugu Mstn gene was similar to that identified in the medaka TILLING library, demonstrating that we were able to establish an effective protocol of the TILLING method in fugu.
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