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RT-PCR analysis of different development stages/tissues (embryos, male and female larvae, adult heads and adult bodies) with primers located in the 5' UTR and exon 7 produced a single product of about 840 bp in each case, suggesting that the gene is not alternatively spliced (data not shown).
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Comparisons between related Bacillus strains show a nonconserved genomic distribution with the repeat sequence flanked by 5 bp DRs in each case.
In contrast to the evidence from human [ 1] and bank vole, annotations of various rodent mt genome sequences in GenBank (including the reference mouse mt genome) include complete TAG stop codons in the CDS of both nad1 and nad2 that overlap the downstream trnI and trnW by 2 bp (AG) in each case (Fig. 1).
These conversion events (which included ∼130 bp of upstream sequence in each case) extended far enough to restore the CCAAT promoter element, thereby promoting the upregulation of HBD.
The failure to synthesise peramine is consistent with the 1223 bp 3'-terminal deletion of the perA gene in all individuals, as well as the premature stop codon at coordinate 2923 bp in all but E1, in each case generating a non-functional gene copy.
With the rpoB PCR, 6 (24%) of the 25 specimens from sarcoidosis patients yielded a product of 360 bp, which by sequence analysis in each case was most consistent with M. tuberculosis.
The cDNA of Colias cytosolic PEPCK includes 1929 bp, 643 codons, and the mitochondrial form includes 1896 bp, 632 codons (omitting the stop in each case).
A 100-bp DNA ladder was used in each case for convenience.
A 'meganuclease', such as I-SceI, that recognizes and cuts longer sequences (18 bp in the case of I-SceI) than those cut by commonly used restriction enzymes.
The length of the two chromosome 3 short arms studied is 17,111,432 bp in the case of O. glaberrima and 19,401,704 in that of O. sativa.
The shorter read length (33 bp) in the case of the HI mutant was compensated for by sequencing two Illumina lanes instead of one.
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