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Each amplified miRNA included the flanking genomic sequences of 110 bp from both sides.
Subsequently, the QTL7.2 support interval was estimated to be 5,191,605 bp from both sides of the marker.
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It was identified that the interval supporting QTL1.2 was about 1,794,113 bp (7.5 cM * 239,215 bp) from both side of the SNP marker.
Thus, the interval supporting the bin 6.04 QTL was about 1,837,208 bp (7.5 cM * 244,961 bp) from both side of the SNP marker.
Subtract b from both sides.
For promoter associated peaks, 250 bp sequences from both sides of peak summits have been retrieved.
For promoter associated peaks, 250 bp sequences from both sides of peak summits were retrieved.
The library insert size ranged for 300 500 bp and fragments were sequenced from both sides yielding two times 100 bp mated sequences.
CNPs were defined as groups of at least two polymorphisms with a distance of less than 200 bp between two consecutive polymorphic sites, separated from the next sequence by a difference greater than 200 bp on both sides.
SNPs were defined as polymorphisms separated from the next nucleotide polymorphism by a difference greater than 200 bp on both sides.
Read pairs were then synchronized such that all read pairs with sequences of at least 35 bp on both sides after quality trimming were retained and any singleton read resulted from reads trimming were removed.
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