Sentence examples for bp fragments using a from inspiring English sources
Purified DNA was mechanically sheared into 350 700 bp fragments using a Hydroshear apparatus fitted with a custom short shearing assembly (GeneMachines).
However, given this unique opportunity to re-test the exact material originally evaluated by Gill et al. [3], we were successfully able to amplify 444 bp and 440 bp fragments using a classic aDNA amplification strategy (increased cycle number, additional BSA, and additional polymerase), see Figure 4.
One μg genomic DNA was sheared into ~200 bp fragments using a Covaris S2 sonication system (Covaris, Woburn, MA).
Briefly, the DNA was sheared to 350 450 bp fragments using a Covaris E210 and purified using AMPure XP Beads according to the manufacturer's instructions.
Cells were lysed by bead beating (6 × 2 min on, 2 min off) and chromatin was sheared to 300-600 bp fragments using a Misonix Sonicator 3000.
After PCR amplification of the enriched fragments, we selected the 220 320 bp fragments using a Gel Extraction kit (28706; Qiagen).
The crosslinked chromatin was sonicated to shear it into 400-bp fragments using a Bioruptor sonicator (Diagenode, Denville, NJ, USA).
For each NGS sample, 1 3 µg of genomic DNA was sheared to <700-bp fragments using a Bioruptor sonicator (Diagenode).
DNA was sheared to 150 200-bp fragments using a Covaris S2 ultrasonicator (Covaris, Woburn, Massachusetts, USA) and directly used to prepare libraries with the NEBNext Ultra DNA Library Prep Kit (#E7370S; New England Biolabs) and multiplex barcodes (#E7335S and #E7500S; New England Biolabs) with nine rounds of PCR.
In addition, 1-µg portions of metagenomic DNA were sheared to approximately 200-bp fragments using a Covaris-S instrument (Covaris, Woburn, MY, USA), and adapter sequences were ligated to both ends of the DNA fragments to generate a paired-end library.
This assay combines PCR amplification of the small subunit (ssu) ribosomal RNA gene (491 500 bp fragments) using genus specific primers, followed by a multiplex species-specific ligation detection reaction (LDR).
