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In contrast, one PCR product of 356 bp corresponding to a fragment of N gene was obtained for IBV strains and one PCR product of 568 bp corresponding to a fragment of S gene was obtained for BCoV.
Bioinformatic analyses revealed an open reading frame of 1551 bp corresponding to a primary translation product of 516 amino acids.
The full-length cDNA of the PmVCP mRNA consisted of 2724 bp containing an ORF of 2367 bp corresponding to a deduced polypeptide of 788 amino acids.
In trench T2, however, human skeletal remains of a middle aged male, which yield a radiocarbon age of 1159 ± 28 BP (corresponding to a mean calendar age of 791 AD), were found within a faulted alluvial layer at a depth of 60 70 cm from the surface.
Fragments of approximately 300 bp corresponding to a putative figla product were obtained using S. alburnoides (AA) and S.pyrenaicus (PP) as templates.
The full-length intronless sequence of 1008 bp, corresponding to a putative lamprey receptor of 336 amino acids was obtained.
Similar(39)
These sequences depart from a central Q node (Figures 1 and 2) by 5.2 5.6 substitutions in the coding region (15,428 bp), corresponding to an estimated divergence time of 16 18 ky (Table 3) when using the evolutionary rate estimate of 2.043±0.099×10−8 base substitutions per nucleotide per year [9].
Size selection was performed using a 2% agarose gel and a slice was excised at 200 bp corresponding to an insert size of 140 bp.
The mean insert size across the 16 samples was 309 bp, corresponding to an unsequenced insert between reads of ∼109 bp.
The average length of the CDS was 938 bp, corresponding to an average polypeptide length of 313 aa, which was also shorter than those of Arabidopsis, rice, and soybean, and longer than poplar and maize [ 26, 27] (Table 2).
The average length of the open reading frame was 718 and 711 bp, corresponding to an average polypeptide length of 239 and 237aa, respectively, which was also shorter than those of Arabidopsis, rice, and soybean, and longer than poplar and maize [ 39, 40].
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