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L and BP cells, however, might suffer from intrinsic stress.
However, the energy flux in L and BP cells was similar to that in K cells, although L and BP cells had much higher rates of cell division.
However, L and BP cells were ∼25% smaller in diameter than K cells (Figure 2A).
In other words, L and BP cells possessed about half the mass of K cells.
BP cells were derived from L, by benzo[a]pyrene treatment.
Since no increase in energy production/utilization could be detected in L and BP cells in spite of their enhanced growth rate, we sought the energy source for the enhanced growth rate exhibited by L and BP cells.
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Another example is the module "BP: cell cycle arrest" in "CC: cytoplasm".
We identified 35, 7 and 19 gene categories, at "level 3", within the biological process (BP), cell compound (CC) and molecular function (MF) classes, respectively (Additional file 4).
In all, we identified 313 brain (61 bp ~ 1,304 bp, 50,324 bp in total, N50 contig size is 195 bp) and 173 (61 bp ~ 821 bp, 29,664 bp in total, N50 contig size is 194 bp) cell line novel transcript contigs that are unalignable to GRCh37, RefSeq genes and EST sequences (Additional files 8 and 9).
We demonstrated that the minimal FPGS promoter region previously described in CCRF-CEM is not sufficient to effectively drive FPGS transcription in NALM6 cells, suggesting that different regulatory elements are required for FPGS gene expression in Bp-cells.
These low levels of FPGS-luciferase activity observed with our constructs in NALM6 vs. CCRF-CEM cells, lead us to conclude that the minimal promoter region is not sufficient to effectively drive FPGS transcription in Bp-cells (NALM6).
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