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Chromosome 1 overgos had an average of 3,610,538 bp between any two overgos in a row pool and 4,243,653 bp between any two overgos in a column pool, while the chromosome 3 overgos had an average spacing of 2,340,440 bp between any two overgos in a row pool and 1,498,379 bp between any two overgos in a column pool.
To ensure that large indels (≥100 bp) between any two cultivars were not due to misassembled contigs, we mapped all Illumina reads of the Kasalath genome to its pseudomolecule sequences by using BWA to confirm that the boundaries of the insertions (Supplementary Fig. S2A) and deletions (Supplementary Fig. S2B) were covered by at least five overlapping reads stepped over by their paired sequences.
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A sample size of 346 (allowing 15% dropouts) will confer 90% power to detect a 3 mm Hg HBPM average systolic BP difference between any two drugs.
Secondly, the size of amplified fragments was between 191 bp and 427 bp, and the difference between any two fragments surpassed 100 bp, not only reducing the amplification efficiency imbalance problem [ 26], but also making the bands easy to distinguish on the electrophoretogram.
The 6 fingers contained 3ZfFLO11/F2 in the N-terminus and 3ZfFLO11/F1 in the C-terminus, with a central linker containing TGGGGSGGSERP, able to span a gap of 2-bp between two 9-binding sites (3-bp gap, if the cross-strand position is counted as being in the gap; Moore, M., unpubl.; Figure 1D).
Because it is difficult to measure aortic pressure invasively during CMR, this will likely require a validation in a relatively large number of subjects with CMR and invasive pressure measurements performed on two separate occasions, with correction for any differences in BP between the two occasions.
ΔBP was defined as systolic BP minus SBP2, because of the difference in systolic BP between the two groups.
However, in covariate-adjusted analyses we did not find any significant difference in BP between the two groups.
The first IAC with a size of approximately 200 bp was constructed by deleting a part of the amplicon of the original target DNA (500 bp) between the two primer sites to produce an IAC smaller than the target DNA.
The lengths (in bp) of sequences showing identity to part or all of the candidate sequence, as well as the separation (in bp) between the two identical sequences, were used in the calculations.
SV4 and SV5 were analyzed together, due to only a minor difference (15 bp) between the two variants.
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