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The genome of D. phosphitoxidans contains one circular chromosome (4,998,761 bp) and a small plasmid (7,714 bp).
The cemA-coding sequence of P. verticillata contained a poly(A) sequence (9 bp) and a small single repeat (SSR CAA unit (fig. 4 A).
The genome of A. orientalis HCCB10007 comprises two replicons, a large circular chromosome (8,948,591 bp) and a small, dissociated circular plasmid (33,499 bp).
Lastly, a large central region in L7rfo (13,722 bp) and a small region upstream of PPR-1 (1,163 bp) showed no clear similarity with the D81Rfo sequence.
The circular double-stranded cpDNA of P. verticillata (157,379 bp) consists of two inverted repeat regions each of 28,373 bp, a large single copy of 82,726 bp, and a small single copy of 17,907 bp.
For these reasons, we chose the S. cerevisiae genome as the model system in our study because it contains relatively short intergenic regions (median length shorter than 400 bp) and a small number of introns [ 66].
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A PCR product was generated using primers 5′-aaatcaactcaatcatgtgaaagc-3′ (forward) and 5′-ggtgaccagcattttatggag-3′ (reverse), which give a normal-sized product of 1752 bp and a smaller product of 853 bp (see Figure 1).
In this case, PCR amplification originated two fragments: the expected wild-type product (322 bp) and a smaller mutant product (304 bp), allowing the independent sequencing of both alleles (data not shown).
The genome of Arthrobacter sp. Rue61a comprises a circular chromosome of 4,736,495 bp, one circular plasmid pARUE232 of 231,551 bp, and a smaller linear plasmid pARUE113 of 112,992 bp.
An approach designed by Carter et al. used a similar scheme to produce a larger amplicon for the Hp allele (4370 bp) and a smaller amplicon for the Hp allele (3000 bp) [ 15].
It consists of a circular chromosome of 1,673,997 bp and of a small plasmid of 6,032 bp carrying nine putative genes.
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