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Indeed, we observed a positive correlation between reductions in GS and BP and a negative correlation between IF and BP.
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Due to the normal daytime BP being more than the nighttime BP, a negative day-night ratio was classified as "reverse dipping".
Zero phase lag signifies synchronous fluctuations, whilst positive phase suggests CBFV leading BP, and negative phase suggests BP leading CBFV.
The SYT positive control reaction generated the expected product of 200 bp, and the negative control yielded no product.
For proline, a compatible osmolyte [ 67], one 6-fold up-regulated UniTag with homology to a proline/betain transporter (STCa-24308) as well as moderate down- and up-regulation of two 26 bp tags representing a negative regulator for proline accumulation (proline dehydrogenase; STCa-8454, STCa-8455) were revealed.
We extended the investigation to a larger minicircle (MCsp2) that contained 1752 bp and an average of 11.2 negative supercoils.
SAMTOOLS [ 17] was used to remove reads with low alignment quality (<20) before peak calling with MACS [ 18] v 1.41, using a fixed fragment size of 160 bp and an IgG sample as negative control.
Figure 2 depicts a representative gel showing a positive (136 bp) and negative pleural fluid and lymph node aspirate.
Conversely, NFAT can activate RCAN1 isoform 4 gene expression via several responsive elements in the promoter region of −350 166 bp, thus forming a negative feedback loop.
The levels of supercoiling produced in this process are prodigious, amounting to a positive and a negative supercoil for every 10 bp transcribed.
Differentially expressed genes were selected based on an absolute aFD value exceeding 1.5, where positive aFD values indicate higher expression at 'peak' BP and negative aFD values indicate higher expression 'trough' BP in the Schlager hypertensive mouse.
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