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The human MR-GEF mRNA comprises a short 5' untranslated region (UTR), an ORF of 1740 bp and a long 3' UTR of more than 3 kb that generates a single transcript (see Figure S1) [17].
Analysis of the different PCR products, confirmed the occurrence of a short (279 bp) and a long (399 bp) transcript.
Although tammar exon 5 was identified using the "Infernal" program, it showed very low sequence conservation (only 53 bp) and a long branch length in the phylogenetic tree.
A shotgun paired-end library with a fragment size between 150 to 500 bp and a long jumping distance mate-pair library with an insert-size between 2 to 10 kb were constructed.
Two DNA-libraries were constructed by Eurofins Genomics (France) using the TruSeq(TM) SBS v3 sequencing kit: a shotgun (SG) paired-end library with a fragment size between 150 to 500 bp and a long jumping distance (LJD) mate-pair library with an insert-size average of 5.7 kbp.
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We applied the NaS approach using the previously described 66,492 MinION® reads and it generated 11,275 NaS reads (with a cumulative size of 82.8 Mb, a N50 of 11,292 bp, and a longest read size of 59,863 bp, Table 2).
The mitogenomes of Thitarodes renzhiensis and T. yunnanensis are 16,173 bp and 15,816 bp long with an A + T content of 81.28 % and 82.34 %, respectively.
This suggests the presence of two distinct classes of introns: short introns, which make up the majority of the sample, with a modal value at 55 bp, and long introns, with a modal value of 80 bp.
Former experiments conducted in the directly visually controlled condition showed that mean throughput was 8.43 bps for a laparoscopic instrument pointing [ 43] and was 8.06 bps for a long dowel pointing [ 57].
The described PCR yields a long (529 bp) and a short (486 bp) fragment which were visualized with UV after 2 h separation at 180 V on a 2.5% agarose gel containing GelRed®.
These experiments detected a long (~280 bp) and a short (~180 bp) fragment, which were individually characterized by nucleotide sequence analysis to encode mitochondrial/cytosolic and cytosolic FPGS, respectively.
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