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These elements have two salient features: a size around 550 bp and a high G+C content (mean value 66.5%).
The internal sequence was corrupted by several stop codons introduced by indels and/or base substitutions, although its flanking 5'- and 3'-LTRs had an identical size (381 bp) and a high degree of sequence identity (99.7%).
There is substantial sequence divergence from the A-genome at a frequency of 1 homozygous SNP per 23.1 bp, and a high degree of heterozygosity corresponding to one heterozygous SNP per 55.9 bp.
Among the ALDH2*2 allele carriers, the frequency of drinkers was comparable between the subjects with high and low GGT levels, and a high GGT level was significantly associated with multiple factors, including an ever-smoking habit, hypertension, high systolic and diastolic BP and a high BMI.
Among the ALDH2*2 allele carriers, the frequency of drinkers was comparable between the subjects with high and low GGT levels (Table 2), and a high GGT level was significantly associated with multiple cardiovascular risk factors, such as an ever-smoking habit, hypertension, high systolic and diastolic BP and a high BMI (Table 3).
XbaI produced a ladder-like pattern (the shorter fragment at about 350 bp, here referred to as XbaI350), while PstI produced two main fragments, a small one of 530 bp and a high molecular weight one of 5.5 kb (here referred to as, respectively, PstI530 and PstI5500).
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The b6 f complex comprises seven subunits: a cyt b6 with a low-potential (bp) and a high-potential (bn) heme, a cyt f, a Rieske iron sulfur protein, subunit IV, and three low molar mass (∼4 kDa) transmembrane subunits.
Intermediate and high amylose varieties were genotyped for SNP status at exon 6 (A/C) using allele-specific primers (5′-CCC ATA CTT CAA AGG AAC ATA-3′, 5′ - GGT TGG AAG CAT CAC GAG TT – 3′ and 5′ - TCT TCA GGT AGC TCG CCA GT – 3′), where a product size of 292 bp indicates C (intermediate amylose) and products of 200 and 292 bp identify an A (high amylose).
Contigs were merged if they had a common seed larger than 30 bp and with a high alignment score.
Table 1 shows runtimes, simulating sequencing of the low diversity dataset with 400 bp reads and a high error rate of 5% to various depths.
After trimming vector contamination, low quality bases, and eliminating trimmed sequences with length less than 100 bp, three libraries resulted in 58,842 high-quality EST sequences with an average read length of 755±171 bp and an average high quality base pairs per read of 670±181.
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