Sentence examples for box deletion from inspiring English sources

To investigate the involvement of the SOCS box of Rab40C in Varp binding, we produced a SOCS box deletion mutant, named Rab40C-ΔSOCS, and evaluated its Varp binding ability by co-immunoprecipitation assays as described above.

To examine Aurora-A ubiquitylation specifically by the CUL3-KLHL18 ligand, anotnot by APC/C, we constructed a C-terminal D box deletion mutant of Aurora-A (Aurora-A ΔD) for in vivo ligase assays.

pcDNA3 cyclin D1 T286andnd cyclin D1 ΔD mutants, as well as F-box deletion (ΔF) mutant forms of FBXW8 and SKP2 were generated by using site-directed mutagenesis (QuickChange and ExSite, Stratagene).

We did not observe an increase for any of the mutants in the proportion of cells with a diffuse nuclear pattern of Pc2 localization, as seen with the C-box deletion.

The F-box deletion ΔF) mutant form of FBXW8 is considered to be a dominant-negative because it can bind to cyclin D1 but barely associates with SKP1, CUL1 and CUL7 (Fig. 4B, lane 3), and therefore does not bring cyclin D1 into the ubiquitin-proteasome pathway.

To test the latter, we transformed in epe1∆:: kanMX into the reporter strain containing the IR-L B-box deletion.

EndoG WT was ubiquitinated by CHIP WT but not by CHIP K30A or the U-box deletion mutant.

Both TPR and U-box deletion constructs of CHIP were unable to reduce BACE1 protein level (Fig. 1E).

A swi6∆:: kanMX construct was transformed into reporter strains containing either the wild-type IR-L or the IR-L B-box deletion mutation.

If Epe1 and TFIIIC worked in two separate pathways, then factors that function only in one of these two pathways would be anticipated to have distinct phenotypes in the wild type, epe1Δ, or the B-box deletion strains.

Interestingly, we found several peptides corresponding to ubiquitin in our USP52 immunoprecipitates, and transiently overexpressing USP52 harbouring a Cys-box deletion mutation in the UCH (ubiquitin C-terminal hydrolase) domain resulted in a decrease in HIF1A protein (results not shown), suggesting that this domain may be important in protecting HIF1A mRNA in P-bodies.