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A recent bovine study has suggested that an increase of IGF1 bioavailability has a negative effect on oocyte developmental competence [ 44].
The low reported LH concentration in the bovine study may reflect the short half-life of only 14 min [ 40] which makes accurate measurements of the LH concentration more difficult.
Recently, Huang and Khatib [ 21] published the first bovine study to use RNA-seq, focusing on the transcriptomic landscape of embryos and concluded that this technology was highly suitable for future gene expression studies in the bovine, a conclusion in line with other groups [ 22].
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All bovine studies were Columbia Institutional Animal Care and Use Committee (IACUC -exempt.
In addition, the LVOT on the right 4th ICS was orientated differently from other bovine studies [ 8, 9, 17].
In bovine studies, it has been demonstrated that BCG protection would be enhanced with repetitive immunization at short intervals [ 72].
The results are in agreement with other human and mouse models using M. tuberculosis [ 52- 55] and bovine studies using MAP.
Furthermore, no bovine studies to date have applied next-generation sequencing (NGS) to examine global miRNA expression in immunity and infection in vivo.
Cholinergic agents via the NO/cGMP pathway have been shown to inhibit CP Na,K-ATPase activity in bovine studies [ 33].
In bovine, studies have reported that the expression of SCD gene is regulated by the transcription factors SREBP1 [ 28, 29], PPARα [ 29] and PPARγ [ 28, 29].
Similar RNA-Seq read mapping rate and the number of genes identified were obtained in other RNA-Seq bovine studies [ 33- 38].
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