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Glutamate is the major excitatory neurotransmitter at the type I bouton of the Drosophila larval NMJ (Brunner and Okane, 1997; Collins and DiAntonio, 2007).
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Immunostaining of the NMJ using Mnb antibody that had been preabsorbed with the immunizing protein (Fig. 1c) further confirms the specificity of Mnb staining and indicates Mnb is enriched in the presynaptic boutons of the Drosophila NMJ, consistent with a recent report that demonstrated Dyrk1A is found in a subset of motor neurons and present in the mouse NMJ24.
Thus, the ARD distribution closely matches that of the boutons of the cha-Tan neurons, but it is not clear what neuron type(s) expresses the receptor.
Rather we noted a close association between the boutons of the rdl-Tan neurons and GBR-IR signal in C2 varicosities and/or large boutons of Cha-Tan neurons (Fig. 9A,C).
We could detect vAChT-immunolabeling in basal processes likely to represent collaterals of L4 neurons (Fig. 5D) and in the dilated boutons of the Cha-Tan neurons (Fig. 6D).
Unlike the immature morphology of their dendrites, the mossy fiber giant boutons of the newborn neurons are similar to those of mature dentate granule cells (Figure 6b,e), providing direct evidence to support the notion that dendrites of developing dentate granule cells mature after their axons reach the CA3 region [45].
Bouton number total number of mapped boutons of the respective neuron within a layer IV barrel.
GFP RSK localizes presynaptically in boutons of the NMJ (Fig. 1).
(D and F ) Electron micrographs of boutons of the same classical (A and D ) and horizontal (B and F ) BCs innervating somata in the CA1 pyramidal layer.
A direct test of these possibilities has been lacking because it has not been possible to record from the type 1 presynaptic boutons of the Drosophila NMJ, which are embedded within the postsynaptic muscle cell.
Intraparenchymal Sap injections in the NBM and MS induced an extensive loss of ChAT-IR in the synaptic boutons of the neocortex and hippocampus, as demonstrated by a strong reduction in ChAT expression.
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