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We highlight recent studies in zebrafish and Xenopus linking Balbiani body to meiotic chromosomal bouquet formation and implicating amyloid aggregation of Buc/Velo1, a key Balbiani body component.
Thus, Rap1 is able to retain bouquet formation under heavily phosphorylated status.
Thus, rap1-DD337AA is a meiosis-specific loss-of-function mutation, and negatively charged aspartates at positions 337 and 338 are crucial for bouquet formation.
Cytological studies show that telomere clustering is Ndj1p dependent, making NDJ1 the first gene linked to bouquet formation in any organism.
Next, we investigated the spatial distribution of the parental genomes from leptotene to the telomere clustering (bouquet formation) at the onset of zygonema more closely.
In contrast to Schizosaccharomyces pombe and multicellular eukaryotes, meiotic bouquet formation in S. cerevisiae is actin-, notubulinin dependent (Scherthan et al. 2007; Koszul et al. 2008).
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Thus, the formation of the bouquet provides the physical opportunity for concerted evolution to occur between similar sequences on non-homologous chromosomes (such as centromeres, and the associated rDNA genes [ 71, 72, 76]).
One long-standing hypothesis is that formation of the telomere bouquet at the onset of zygotene is instigating homologue pairing (Scherthan 2001).
The formation and dynamics of the bouquet configuration of meiotic chromosomes contribute to proper homologous chromosome pairing, synapsis, recombination, and segregation [ 45- 50].
It is generally concomitant with formation of the "meiotic chromosome bouquet," a special chromosome configuration in which one or both chromosome ends attach to the nuclear envelope and become concentrated within a limited area.
As described in Berríos et al. (2010), the cluster formation (disaggregation of the prophase bouquet with CTC's attached to the nuclear envelope) can be modeled as a placement of single CTC's upon the inner surface of the nuclear envelope by means of a Laplace mechanism.
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