Puncta with any portion appearing outside the boundaries of the terminal were removed from the analysis sample, as were those falling within the first and last optical sections of the stack as deconvolution is not reliable at these positions.
Further, in order to overcome the problem of decoupled method that the boundary of the device's terminal must be connected to a voltage source, we present a partial decoupled equivalent-circuit model (PDM) with levelized incomplete LU factorization.
The view of safety experts is that the boundaries of the Los Angeles terminal control area are extremely complicated and should be simplified.
This suggests that the functional domain boundaries of the N-terminal NES in FOXO1a may be redefined from residues 1 and 50 to residues 8 and 32.
This enabled us to precisely define the boundaries of the C-terminal toxin domains and use them as seeds in iterative sequence profile searches with the PSI-BLAST and JACKHMMER programs.
This region begins at the N terminal boundary of the Pfam GTP_EFTU domain and ends at the C terminal boundary of the predicted D2 domain, or since not all trGTPases have a predicted Pfam D2 domain even though they do in fact carry it, 100 amino acids after the C terminal boundary of the GTP_EFTU (the average length of D2 is 103 amino acids).
N-glycosylation sites are found in each of the proteins; within both PNPLA2/ATGL and PNPLA4, one N-glycosylation site is located at the N-terminal boundary of the patatin domain, and in PNPLA9 it is close to the C-terminal boundary, respectively.
The C-terminal boundary of the intein, S472, immediately follows the conserved His-Asn residues often associated with the C-terminal intein-extein boundary [ 51].
The N-terminal boundary of the PH CC Ex regions was determined by comparison with other canonical PH domains, whereas the C-terminal domain boundary was not determined uniquely using sequences.
