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The exon intron boundaries were verified using the expressed sequence tag sequence for dmtA and by RT-PCR for stlB and chlA homologs.
Intron/exon boundaries were verified using the ghmme3 program of genemark [ Arabidopsis splice junctions were used as a reference (Besemer and Borodovsky 2005)].
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Anatomical boundaries of specific nuclei and brain regions were verified using the Rat Brain atlas by Paxinos and Watson [27].
The medial boundaries were determined on each slice using cerebro spinal fluid as a landmark; lateral limits were verified using FA maps to exclude the internal capsule.
The results were verified using a breath test.
The plasmids were verified using sequencing.
Protein topologies were verified using SMART-EMBL.
Gene annotations were verified using VEGA [ 46].
Associations were verified using multiple logistic regression.
Results were verified using two independent methods.
Detected mutations were verified using direct sequencing.
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