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The flow regime boundaries were defined using the conventional methods of flow regime demarcation such as the change in the slope of the overall gas holdup and the drift flux plot.
Nuclei were defined using the DAPI channel and cell boundaries were defined using E-cadherin-647 nm channel.
169 sequences used in Lin et al.[ 16] were collected and recA/RAD51 domain boundaries were defined using NCBI CDD default settings [ 21].
Intron exon boundaries were defined using the Transdecoder package r2013-11-17 (http://transdecoder.sourceforge.net/, last accessed September 16, 2015) (supplementary fig. S1, Supplementary Material online).
For each pattern obtained from this set, the gene expression boundaries were defined using the following algorithm that searched for the maximum or minimum positions at which expression levels of the different TFs were higher or lower than each other; specifically, the Nkx2.2 (N)/Olig2 (O) boundary was defined as: mean[max(N>O in region N>I), min(O>N in region O>I)].
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In the third stage, districts were arbitrarily identified within each urban and rural region, boundaries being defined using local maps or in consultation with the local health workers.
The tRNA gene boundaries and folded structures were defined using tRNAscan-SE (http://lowelab.ucsc.edu/tRNAscan-SE/) [31] with the following parameters: (i) search mode – organellar; (ii) search type – cove only; (iii) cove cut off score −15; (iv) translation code used – invertebrate mitochondrial; (v) maximum intron length – 40; and (vi) pseudogene checking – disabled.
Given a gene, the genomic boundaries of its DHS-based regulatory block were defined using the most distal DHS connected to that gene.
For each connexion boundary, a "boundary structure" (whose characteristics are defined using a physical criterion) is associated.
EWT acts like a filter bank in which the support boundaries of the filter are defined using OMA.
For design-based stereology, the volume of interest (VOI) is defined using reproducible anatomical boundaries.
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