Although, in principle, the FACS selection should work such that a cell with expression level anywhere within the selection window has 100% probability to be selected, and 0% probability to be selected if the cell's expression is anywhere outside the selection window, it is unrealistic to assume that the boundaries of the selection window are so precisely defined in practice.
Considering the boundaries of the selection window, it need to be predictable on the basis of data obtained by clinical microbiological laboratories.
The data in Figure 2 demonstrate that agar plate determinations of MIC99 and MPC fit well with the boundaries of the selection window seen in vivo at the site of infection for cefquinome.
T>MPC is probably the appropriate parameter for the upper boundary of the selection window.
Herein, the T>MIC99 (for time-dependent drugs) or AUC24 h/MIC99 (for concentration-dependent drugs) can be used to define the lower boundary of the selection window in PK/PD combined with MSW model.
The boundaries of the mutant selection window were determined to be 0.06 μg/mL (MIC99) and 0.16 μg/mL (MPC) by agar plate assays, which were the average values determined in the five independent experiments.
The analysis of the papers showed that within the constraints of the ISO standards, there is variability in the definition of the functional unit and the system boundaries, the selection of the impact assessment methodology and the procedure followed for interpreting the results.
Presumably, selection against boundary changes will be inversely related to the availability of true alternative boundaries, and the overall efficacy of selection in general (effective population size).
The evolution of species boundaries and the relative impact of selection and gene flow on genomic divergence are best studied in populations and species pairs exhibiting various levels of divergence along the speciation continuum.
The necessities of boundary layer meshing, selection of appropriate solver, impulse simulation, requirement of non-reflecting velocity inlet, simulation of two-load boundary condition, and role of microphone spacing have been discussed in the context of TL evaluation in the presence of mean flow.
